In vitro and in vivo activities of tigecycline-colistin combination therapies against carbapenem-resistant Enterobacteriaceae

Jonathan W Betts; Lynette M Phee; Michael Hornsey; Neil Woodford; David W Wareham

doi:10.1128/AAC.02449-14

In vitro and in vivo activities of tigecycline-colistin combination therapies against carbapenem-resistant Enterobacteriaceae

Antimicrob Agents Chemother. 2014 Jun;58(6):3541-6. doi: 10.1128/AAC.02449-14. Epub 2014 Mar 31.

Authors

Jonathan W Betts¹, Lynette M Phee², Michael Hornsey³, Neil Woodford⁴, David W Wareham⁵

Affiliations

¹ Antimicrobial Research Group, Blizard Institute, Queen Mary University of London, London, United Kingdom.
² Antimicrobial Research Group, Blizard Institute, Queen Mary University of London, London, United Kingdom Division of Infection, Barts Health NHS Trust, London, United Kingdom.
³ Department of Life Sciences, University of Roehampton, London, United Kingdom.
⁴ Antimicrobial Research Group, Blizard Institute, Queen Mary University of London, London, United Kingdom Antimicrobial Resistance Healthcare Associated Infections Reference Unit, Public Health England, Colindale, London, United Kingdom.
⁵ Antimicrobial Research Group, Blizard Institute, Queen Mary University of London, London, United Kingdom Division of Infection, Barts Health NHS Trust, London, United Kingdom d.w.wareham@qmul.ac.uk.

Abstract

We assessed the activity of tigecycline (TGC) combined with colistin (COL) against carbapenem-resistant enterobacteria. Synergy occurred in vitro against the majority of isolates, with the exception of Serratia marcescens. In a simple animal model (Galleria mellonella), TGC-COL was superior (P < 0.01) in treating Escherichia coli, Klebsiella pneumoniae, and Enterobacter infections, including those with TGC-COL resistance. Clinical studies are needed to determine whether TGC-COL regimens may be a viable option.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology*
Carbapenems / pharmacology
Colistin / pharmacology*
Disease Models, Animal
Drug Resistance, Bacterial
Drug Therapy, Combination
Enterobacter / drug effects
Enterobacteriaceae / drug effects*
Enterobacteriaceae Infections / drug therapy*
Enterobacteriaceae Infections / microbiology
Klebsiella pneumoniae / drug effects
Microbial Sensitivity Tests
Minocycline / analogs & derivatives*
Minocycline / pharmacology
Moths
Tigecycline
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Carbapenems
Tigecycline
beta-Lactamases
Minocycline
Colistin