Antisense long non-coding RNA PCNA-AS1 promotes tumor growth by regulating proliferating cell nuclear antigen in hepatocellular carcinoma

Sheng-Xian Yuan; Qi-Fei Tao; Jie Wang; Fu Yang; Lei Liu; Li-Li Wang; Jin Zhang; Yuan Yang; Hui Liu; Fang Wang; Shu-Han Sun; Wei-Ping Zhou

doi:10.1016/j.canlet.2014.03.029

Antisense long non-coding RNA PCNA-AS1 promotes tumor growth by regulating proliferating cell nuclear antigen in hepatocellular carcinoma

Cancer Lett. 2014 Jul 10;349(1):87-94. doi: 10.1016/j.canlet.2014.03.029. Epub 2014 Apr 2.

Authors

Sheng-Xian Yuan¹, Qi-Fei Tao², Jie Wang³, Fu Yang⁴, Lei Liu⁵, Li-Li Wang⁶, Jin Zhang⁷, Yuan Yang⁸, Hui Liu⁹, Fang Wang¹⁰, Shu-Han Sun¹¹, Wei-Ping Zhou¹²

Affiliations

¹ The Third Department of Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China. Electronic address: yuanshengx@126.com.
² The Third Department of Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China. Electronic address: tqftxl@163.com.
³ Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. Electronic address: msswjw@163.com.
⁴ Department of Medical Genetics, Second Military Medical University, Shanghai, China. Electronic address: yangfusq1997@hotmail.com.
⁵ Key Laboratory of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China. Electronic address: leiliu@sibs.ac.cn.
⁶ The Third Department of Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China. Electronic address: 362769189@qq.com.
⁷ The Third Department of Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China. Electronic address: Zhangjin_sh@126.com.
⁸ The Third Department of Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China. Electronic address: yangyuan.ehbh@gmail.com.
⁹ The Third Department of Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China. Electronic address: liuhuigg@hotmail.com.
¹⁰ Department of Medical Genetics, Second Military Medical University, Shanghai, China. Electronic address: wfsjz70@gmail.com.
¹¹ Department of Medical Genetics, Second Military Medical University, Shanghai, China. Electronic address: shsun@vip.sina.com.
¹² The Third Department of Hepatic Surgery, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai, China. Electronic address: ehphwp3@126.com.

PMID: 24704293
DOI: 10.1016/j.canlet.2014.03.029

Abstract

About 61-72% of transcribed regions possess long noncoding RNAs in antisense orientation (Antisense long noncoding RNAs, aslncRNAs). However, the function of aslncRNAs in HCC remains unclear. We found numerous aslncRNAs were deregulated and might be involved in regulatory gene-net of HCC. The PCNA-AS1, antisense to PCNA, is significantly up-regulated in HCC and could promote tumor growth in vitro and in vivo. The effects of PCNA-AS1 rely on regulation of PCNA via forming RNA hybridization to increase PCNA mRNA stability. We concluded that aslncRNAs might act as upstream regulators in HCC and PCNA-AS1 could serve as a novel therapeutic target.

Keywords: Antisense RNA; Long noncoding RNA; RNA hybridazation; RNA stability; Tumor growth.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinoma, Hepatocellular / genetics*
Carcinoma, Hepatocellular / pathology
Humans
Liver Neoplasms / genetics*
Liver Neoplasms / pathology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Proliferating Cell Nuclear Antigen / genetics*
RNA, Antisense / genetics*
RNA, Long Noncoding / genetics*
RNA, Messenger / genetics
Up-Regulation

Substances

Proliferating Cell Nuclear Antigen
RNA, Antisense
RNA, Long Noncoding
RNA, Messenger