Induction of neutralizing antibodies to influenza A virus H7N9 by inactivated whole virus in mice and nonhuman primates

Weiqi Pan; Ling Han; Zhenyuan Dong; Xuefeng Niu; Zhengfeng Li; Linlin Bao; Chufang Li; Qinfang Luo; Zifeng Yang; Xiaobo Li; Jicheng Huang; Liqiang Feng; Chuan Qin; Nanshan Zhong; Ling Chen

doi:10.1016/j.antiviral.2014.04.003

Induction of neutralizing antibodies to influenza A virus H7N9 by inactivated whole virus in mice and nonhuman primates

Antiviral Res. 2014 Jul:107:1-5. doi: 10.1016/j.antiviral.2014.04.003. Epub 2014 Apr 16.

Authors

Weiqi Pan¹, Ling Han², Zhenyuan Dong³, Xuefeng Niu¹, Zhengfeng Li³, Linlin Bao⁴, Chufang Li¹, Qinfang Luo¹, Zifeng Yang¹, Xiaobo Li⁴, Jicheng Huang⁵, Liqiang Feng³, Chuan Qin⁴, Nanshan Zhong¹, Ling Chen⁶

Affiliations

¹ State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
² Anhui University, Anhui, China.
³ Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China.
⁴ Institute of Laboratory Sciences (ILS), Chinese Academy of Medical Sciences, Beijing, China.
⁵ Health Quarantine Laboratory, Guangdong Inspection and Quarantine Technology Center, Guangzhou, China.
⁶ State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangzhou Institutes of Biomedicine and Health (GIBH), Chinese Academy of Sciences, Guangzhou, China. Electronic address: chen002@gmail.com.

PMID: 24746458
DOI: 10.1016/j.antiviral.2014.04.003

Abstract

We evaluated the immunogenicity of hemagglutinin (HA) in the context of inactivated H7N9/AH/1/13-PR8 whole-virion. At 4weeks after immunization with 15μg HA, mice produced hemagglutination inhibition (HI) titers of 1:192 and neutralizing antibodies of 1:317. Aluminum hydroxide (alum), or a booster immunization, or both increased HI to 1:768, 1:384, 1:896 and neutralizing antibodies to 1:1868, 1:2302, 1:10,000, respectively. Macaques generated HI of 1:190 or 1:360 and virus neutralizing titers of 1:280 or 1:658 at 3weeks after immunization with HA alone or with alum. Sera from immunized mice and macaques protected mice from infection of A/Anhui/1/2013 (H7N9), suggesting an H7N9 vaccine is immunologically feasible.

Keywords: Antibody; H7N9; Hemagglutinin; Influenza; Neutralization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage
Alum Compounds / administration & dosage
Animals
Antibodies, Neutralizing / blood*
Antibodies, Neutralizing / therapeutic use
Antibodies, Viral / blood*
Antibodies, Viral / therapeutic use
Disease Models, Animal
Female
Hemagglutination Inhibition Tests
Hemagglutinin Glycoproteins, Influenza Virus / immunology
Immunization / methods
Immunization, Passive
Influenza A Virus, H7N9 Subtype / immunology*
Influenza Vaccines / administration & dosage
Influenza Vaccines / immunology
Macaca
Mice, Inbred BALB C
Neutralization Tests
Orthomyxoviridae Infections / immunology
Orthomyxoviridae Infections / prevention & control

Substances

Adjuvants, Immunologic
Alum Compounds
Antibodies, Neutralizing
Antibodies, Viral
Hemagglutinin Glycoproteins, Influenza Virus
Influenza Vaccines
aluminum sulfate