Abstract
Alveolar macrophages obtained from healthy rhesus monkeys were infected with SIV in vitro as documented by the appearance of reverse transcriptase activity in the cell-free supernatant, electronmicroscopy, and immunohistochemical methods detecting SIV-related core protein. The results demonstrate permissive infection of alveolar macrophages with SIV in vitro and define a system for studying macrophage-SIV interactions.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bronchoalveolar Lavage Fluid / cytology
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Cells, Cultured
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Immunoenzyme Techniques
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Macaca mulatta
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Macrophages / microbiology*
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Macrophages / ultrastructure
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Microscopy, Electron
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Pulmonary Alveoli / cytology
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Pulmonary Alveoli / microbiology*
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RNA-Directed DNA Polymerase / analysis
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Simian Immunodeficiency Virus / enzymology
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Simian Immunodeficiency Virus / physiology*
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Simian Immunodeficiency Virus / ultrastructure
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Virus Replication*
Substances
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RNA-Directed DNA Polymerase