An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions

Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 2014 Apr 20.

Abstract

We report a protein-fragment complementation assay (PCA) based on the engineered Deinococcus radiodurans infrared fluorescent protein IFP1.4. Unlike previous fluorescent protein PCAs, the IFP PCA is reversible, allowing analysis of spatiotemporal dynamics of hormone-induced signaling complexes in living yeast and mammalian cells at nanometer resolution. The inherently low background of infrared fluorescence permitted detection of subcellular reorganization of a signaling complex expressed at low abundance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism*
  • Cells, Cultured
  • Deinococcus / chemistry
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism*
  • Humans
  • Infrared Rays*
  • Models, Molecular*
  • Spatio-Temporal Analysis*

Substances

  • Bacterial Proteins
  • Fluorescent Dyes