International typing study of Clostridium difficile

Carl E Manzo; Michelle M Merrigan; Stuart Johnson; Dale N Gerding; Thomas V Riley; Joseph Silva Jr; Jon S Brazier; International Clostridium difficile Study Group

doi:10.1016/j.anaerobe.2014.04.005

International typing study of Clostridium difficile

Anaerobe. 2014 Aug:28:4-7. doi: 10.1016/j.anaerobe.2014.04.005. Epub 2014 Apr 21.

Authors

Carl E Manzo¹, Michelle M Merrigan², Stuart Johnson³, Dale N Gerding⁴, Thomas V Riley⁵, Joseph Silva Jr⁶, Jon S Brazier⁷; International Clostridium difficile Study Group

Collaborators

International Clostridium difficile Study Group:
Y Tang-Feldman, M Delmee, M Wilks, M E Mulligan

Affiliations

¹ Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA.
² The Hines Veteran Affairs Hospital, Hines, IL, USA.
³ Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA; The Hines Veteran Affairs Hospital, Hines, IL, USA. Electronic address: stuart.johnson2@va.gov.
⁴ Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA; The Hines Veteran Affairs Hospital, Hines, IL, USA. Electronic address: dale.gerding2@va.gov.
⁵ The University of Western Australia and The Queen Elizabeth II Medical Centre, Perth, Western Australia, Australia.
⁶ University of California, Davis, Sacramento, CA, USA.
⁷ Anaerobe Reference Unit, Public Health Laboratory, University Hospital of Wales, Cardiff, Wales, UK.

PMID: 24768986
DOI: 10.1016/j.anaerobe.2014.04.005

Abstract

We report the results of an international Clostridium difficile typing study to cross reference strain designations for seven typing methodologies and facilitate inter-laboratory communication. Four genotypic and three phenotypic methods were used to type 100 isolates and compare the results to 39 PCR ribotypes identified among the collection.

Keywords: Clostridium difficile; Genotypic typing; PCR ribotyping; Phenotypic typing; REA typing.

Published by Elsevier Ltd.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Typing Techniques / methods*
Clostridioides difficile / classification*
Clostridioides difficile / genetics
Clostridioides difficile / physiology
Genotype
Humans
International Cooperation
Phenotype
Prohibitins