Performance of the CareStart glucose-6-phosphate dehydrogenase (G6PD) rapid diagnostic test in Gressier, Haiti

Am J Trop Med Hyg. 2014 Jul;91(1):77-80. doi: 10.4269/ajtmh.14-0100. Epub 2014 Apr 28.

Abstract

Administering primaquine (PQ) to treat malaria patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency can pose a serious risk of drug-induced hemolysis (DIH). New easy to use point-of-care rapid diagnostic tests are being developed as an alternative to labor-intensive spectrophotometric methods, but they require field testing before they can be used at scale. This study screened 456 participants in Gressier, Haiti using the Access Bio CareStart qualitative G6PD rapid detection test compared with the laboratory-based Trinity Biotech quantitative spectrophotometric assay. Findings suggest that the CareStart test was 90% sensitive for detecting individuals with severe deficiency and 84.8% sensitive for detecting individuals with moderate and severe deficiency compared with the Trinity Biotech assay. A high negative predictive value of 98.2% indicates excellent performance in determining those patients able to take PQ safely. The CareStart G6PD test holds much value for screening malaria patients to determine eligibility for PQ therapy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adolescent
  • Antimalarials
  • Child
  • Contraindications
  • Enzyme Assays*
  • Female
  • Glucosephosphate Dehydrogenase / analysis*
  • Glucosephosphate Dehydrogenase / metabolism
  • Glucosephosphate Dehydrogenase Deficiency / complications
  • Glucosephosphate Dehydrogenase Deficiency / drug therapy
  • Glucosephosphate Dehydrogenase Deficiency / enzymology*
  • Glucosephosphate Dehydrogenase Deficiency / parasitology
  • Haiti
  • Hemolysis
  • Humans
  • Malaria, Vivax / complications
  • Malaria, Vivax / drug therapy
  • Malaria, Vivax / enzymology*
  • Malaria, Vivax / parasitology
  • Male
  • Plasmodium vivax
  • Point-of-Care Systems
  • Predictive Value of Tests
  • Primaquine

Substances

  • Antimalarials
  • Glucosephosphate Dehydrogenase
  • Primaquine