Inhibitory effect of cyclic AMP on phorbol ester-stimulated production of reactive oxygen metabolites in rat glomeruli

Biochem Biophys Res Commun. 1989 Nov 30;165(1):519-25. doi: 10.1016/0006-291x(89)91100-5.

Abstract

Studies were conducted to investigate cross-talk between protein kinase C (PKC) and cyclic AMP (cAMP) pathways using rat glomeruli (Glm). Phorbol 12-myristate 13-acetate (PMA), a PKC activator, stimulated production of reactive oxygen metabolites (ROM) in Glm. Forskolin and dibutyryl cAMP (Bt2cAMP) inhibited production of ROM dose-dependently. In the presence of both Bt2cAMP and 3-isobutyl-1-methylxanthine (IBMX) an additive effect was observed. Forskolin at 10(-4) inhibited translocation of PKC from the cytosol to the membrane. These results demonstrate that cAMP-mediated inhibition can occur at a step distal to PKC activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology*
  • Animals
  • Bucladesine / pharmacology*
  • Colforsin / pharmacology
  • Cyclic AMP / physiology*
  • Kidney Glomerulus / drug effects
  • Kidney Glomerulus / metabolism*
  • Kinetics
  • Luminescent Measurements
  • Male
  • Oxygen / metabolism*
  • Protein Kinase C / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Theophylline / analogs & derivatives*

Substances

  • Colforsin
  • Bucladesine
  • Theophylline
  • Cyclic AMP
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate
  • Oxygen
  • 1-Methyl-3-isobutylxanthine