Detection of Helicobacter pylori in adenoid tissue by real-time polymerase chain reaction

Kulak Burun Bogaz Ihtis Derg. 2014 Mar-Apr;24(2):78-82. doi: 10.5606/kbbihtisas.2014.28445.

Abstract

Objectives: This study aims to investigate the presence of Helicobacter pylori (H. pylori) using the real-time polymerase chain reaction (PCR) method in the adenoid tissues in children undergoing surgical operation due to adenoid hypertrophy.

Patients and methods: Adenoid tissues of 23 children (8 girls, 15 boys; mean age 6.2 years; range 3 to 9 years) who were operated with the diagnosis of adenoid hypertrophy, tonsil + adenoid hypertrophy or tonsil + adenoid hypertrophy + serous otitis media in our clinic between January 2012 and April 2012 were examined.

Results: Of 23 patients, H. pylori was detected in the adenoid tissues of two (8.7%). Regurgitation was present in seven patients. However, no regurgitation was found in H. pylori-positive patients.

Conclusion: In this study the presence of H. pylori in patients with adenoid hypertrophy has been demonstrated using PCR method. To be able to support the hypothesis that H. pylori has a place in etiology of adenoid hypertrophy, multicenter studies are warranted.

MeSH terms

  • Adenoids / microbiology
  • Adenoids / pathology*
  • Adenoids / surgery
  • Child
  • Child, Preschool
  • DNA, Bacterial / analysis
  • Female
  • Helicobacter Infections
  • Helicobacter pylori / genetics
  • Helicobacter pylori / isolation & purification*
  • Humans
  • Hypertrophy / complications
  • Hypertrophy / microbiology
  • Hypertrophy / pathology*
  • Hypertrophy / surgery
  • Male
  • Otitis Media with Effusion / complications*
  • Polymerase Chain Reaction
  • Real-Time Polymerase Chain Reaction

Substances

  • DNA, Bacterial