Abstract
Whole-genome sequencing was used to compare longitudinal isolates of Staphylococcus aureus that developed resistance to oxacillin (MIC up to 16 μg/ml). The mecA gene was absent. A novel 5-bp TATCC frameshift insertion in a gene encoding an ABC transporter similar to that of the teichoic acid translocation ATP-binding protein TagH and a 3-bp GCT nonframeshift insertion in the pdhA pyruvate dehydrogenase gene were detected in the oxacillin-resistant isolates.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
MeSH terms
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ATP-Binding Cassette Transporters / genetics
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Aged, 80 and over
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Anti-Bacterial Agents / pharmacology*
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Bacteremia / microbiology*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial*
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Frameshift Mutation
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Genome, Bacterial
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Humans
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Longitudinal Studies
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Male
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Mutagenesis, Insertional
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Oxacillin / pharmacology*
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Recurrence
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Sequence Analysis, DNA
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / isolation & purification
Substances
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ATP-Binding Cassette Transporters
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Anti-Bacterial Agents
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DNA, Bacterial
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Oxacillin