Abstract
Genome sequencing of carbapenem-resistant Klebsiella pneumoniae isolates from regional U.S. hospitals was used to characterize strain diversity and the bla(KPC) genetic context. A phylogeny based on core single-nucleotide variants (SNVs) supports a division of sequence type 258 (ST258) into two distinct groups. The primary differences between the groups are in the capsular polysaccharide locus (cps) and their plasmid contents. A strict association between clade and KPC variant was found. The bla(KPC) gene was found on variants of two plasmid backbones. This study indicates that highly similar K. pneumoniae subpopulations coexist within the same hospitals over time.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Typing Techniques
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Carbapenems / pharmacology
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Hospitals
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Humans
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Klebsiella Infections / drug therapy
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Klebsiella Infections / epidemiology
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Klebsiella Infections / microbiology
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Klebsiella pneumoniae / classification*
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Klebsiella pneumoniae / drug effects
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Midwestern United States / epidemiology
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Phylogeny
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Plasmids / chemistry*
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Plasmids / metabolism
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Polymorphism, Single Nucleotide
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Polysaccharides, Bacterial / chemistry*
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Polysaccharides, Bacterial / metabolism
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Carbapenems
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Polysaccharides, Bacterial
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beta-Lactamases