Concentration of cytokines in patients with osteoarthritis of the knee and fibromyalgia

Clin Interv Aging. 2014 Jun 12:9:939-44. doi: 10.2147/CIA.S60330. eCollection 2014.

Abstract

Introduction: Fibromyalgia and osteoarthritis may present a relationship with the concentration of cytokines. The aim of this study was to compare the serum concentrations of IL-12p70, tumor necrosis factor, IL-10, IL-6, IL-1β, and IL-8 in patients with knee osteoarthritis and fibromyalgia.

Materials and methods: The study included 53 women (71.2±7.6 years old) diagnosed with knee osteoarthritis with moderate-to-severe pain (visual analog scale >4) for at least 3 months. Sixty women (54.1±8.1 years old) diagnosed with fibromyalgia according to the American College of Rheumatology criteria and with moderate-to-severe pain (visual analog scale >4) also participated in this study. For the dosage of cytokines, blood was collected in the morning: 5 mL from the cubital vein. The material was centrifuged at 4°C, separated into 100 μL aliquots and stored at -80°C until processing. Serum concentrations of the studied cytokines were assessed using the BD Cytometric Bead Array method. Data were analyzed with Student's t-test and the Mann-Whitney U test.

Results: We found higher levels of IL-6, IL-10, and IL-1β in fibromyalgia patients. After adjustment of age as a covariate, there was no statistically significant difference in the concentration of any cytokine between fibromyalgia and knee osteoarthritis patients.

Conclusion: Patients with knee osteoarthritis and fibromyalgia with the same duration and intensity of pain demonstrate similar concentrations of cytokines. Aging may play a role in cytokine profile, a finding not so extensively addressed in the literature and one that should be further investigated.

Keywords: aging; cytokines; fibromyalgia; osteoarthritis.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Cytokines / blood*
  • Female
  • Fibromyalgia / blood*
  • Humans
  • Interleukin-10 / blood
  • Interleukin-12 / blood
  • Interleukin-1beta / blood
  • Interleukin-6 / blood
  • Interleukin-8 / blood
  • Middle Aged
  • Osteoarthritis, Knee / blood*
  • Pain Measurement
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Cytokines
  • Interleukin-1beta
  • Interleukin-6
  • Interleukin-8
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interleukin-12