Accumulation of connective tissue growth factor+ cells during the early phase of rat traumatic brain injury

Diagn Pathol. 2014 Jul 10:9:141. doi: 10.1186/1746-1596-9-141.

Abstract

Background: Glial scar formation is a common histopathological feature of traumatic brain injury (TBI). Astrogliosis and expression of transforming growth factor beta (TGF-β) are key components of scar formation and blood-brain barrier modulation. Connective tissue growth factor (CTGF) is considered a cytokine mediating the effects of TGF-β.

Methods: Here, we studied the CTGF expression in an open-skull weight-drop-induced TBI, with a focus on the early phase, most amenable to therapy.

Results: In normal rat brains of our study, CTGF+ cells were rarely observed. Significant parenchymal accumulation of CTGF+ non-neuron cells was observed 72 h post-TBI and increased continuously during the investigating time. We also observed that the accumulated CTGF+ non-neuron cells were mainly distributed in the perilesional areas and showed activated astrocyte phenotypes with typical stellate morphologic characteristics.

Conclusion: Our observations demonstrated the time-dependent and lesion-associated accumulation of cellular CTGF expression in TBI, suggesting a pathological role of CTGF in TBI.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3963462091241165.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Injuries / metabolism
  • Brain Injuries / pathology*
  • Cicatrix / metabolism
  • Connective Tissue Growth Factor / metabolism*
  • Immunohistochemistry
  • Male
  • Neuroglia / metabolism*
  • Rats
  • Rats, Inbred Lew

Substances

  • Connective Tissue Growth Factor