Human leukocyte antigen class II variants and adult-onset asthma: does occupational allergen exposure play a role?

Eur Respir J. 2014 Nov;44(5):1234-42. doi: 10.1183/09031936.00068014. Epub 2014 Jul 17.

Abstract

Recently, a locus centred on rs9273349 in the HLA-DQ region emerged from genome-wide association studies of adult-onset asthma. We aimed to further investigate the role of human leukocyte antigen (HLA) class II in adult-onset asthma and a possible interaction with occupational exposures. We imputed classical HLA-II alleles from 7579 single-nucleotide polymorphisms in 6025 subjects (1202 with adult-onset asthma) from European cohorts: ECRHS, SAPALDIA, EGEA and B58C, and from surveys of bakers and agricultural workers. Based on an asthma-specific job-exposure matrix, 2629 subjects had ever been exposed to high molecular weight (HMW) allergens. We explored associations between 23 common HLA-II alleles and adult-onset asthma, and tested for gene-environment interaction with occupational exposure to HMW allergens. Interaction was also tested for rs9273349. Marginal associations of classical HLA-II alleles and adult-onset asthma were not statistically significant. Interaction was detected between the DPB1*03:01 allele and exposure to HMW allergens (p = 0.009), in particular to latex (p = 0.01). In the unexposed group, the DPB1*03:01 allele was associated with adult-onset asthma (OR 0.67, 95%CI 0.53-0.86). HMW allergen exposures did not modify the association of rs9273349 with adult-onset asthma. Common classical HLA-II alleles were not marginally associated with adult-onset asthma. The association of latex exposure and adult-onset asthma may be modified by DPB1*03:01.

Publication types

  • Multicenter Study

MeSH terms

  • Adolescent
  • Adult
  • Alleles
  • Allergens / immunology*
  • Asthma / genetics*
  • Asthma / immunology*
  • Asthma / physiopathology
  • Cohort Studies
  • Europe
  • Female
  • Genome-Wide Association Study
  • Genotype
  • HLA-DQ Antigens / genetics
  • HLA-DQ beta-Chains / genetics
  • HLA-DRB1 Chains / genetics
  • Histocompatibility Antigens Class II / genetics*
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Male
  • Middle Aged
  • Molecular Weight
  • Occupational Exposure*
  • Odds Ratio
  • Polymorphism, Single Nucleotide
  • Young Adult

Substances

  • Allergens
  • HLA-DQ Antigens
  • HLA-DQ beta-Chains
  • HLA-DRB1 Chains
  • Histocompatibility Antigens Class II