Portable lactate analyzer for measuring lactate in cerebrospinal fluid (CSF) and plasma - method-comparison evaluations

Arq Neuropsiquiatr. 2014 Jul;72(7):500-5. doi: 10.1590/0004-282x20140076.

Abstract

Increased plasma lactate levels can indicate the presence of metabolic disorders in HIV infected individuals.

Objective: To determine whether a portable analyzer is valid for measuring cerebrospinal fluid (CSF) and plasma lactate levels in HIV infected individuals.

Method: CSF and plasma were collected from 178 subjects. Samples tested by the Accutrend portable analyzer were compared to those tested by a reference device (SYNCHRON LX 20).

Results: The portable analyzer had in plasma sensitivity of 0.95 and specificity 0.87. For CSF the specificity was 0.95; the sensitivity 0.33; the negative predictive value was 95% and the positive predictive value 33%.

Conclusions: These findings support the validity of the portable analyzer in measuring lactate concentrations in CSF that fall within the normal range. The relatively poor positive predictive value indicates that a result above the reference range may represent a "false positive test", and should be confirmed by the reference device before concluding abnormality.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Female
  • HIV Infections / blood*
  • HIV Infections / cerebrospinal fluid*
  • Humans
  • Lactic Acid / blood*
  • Lactic Acid / cerebrospinal fluid*
  • Male
  • Middle Aged
  • Point-of-Care Systems*
  • Predictive Value of Tests
  • Reference Values
  • Reproducibility of Results
  • Time Factors

Substances

  • Lactic Acid