Progress in stacking techniques based on field amplification of capillary electrophoresis

Anal Bioanal Chem. 2014 Oct;406(25):6129-50. doi: 10.1007/s00216-014-8062-7. Epub 2014 Aug 9.

Abstract

Numerous strategies have been developed to mitigate the intrinsic low detection sensitivity that is a limitation of capillary electrophoresis. Among them, in-line stacking is an effective strategy to address the sensitivity challenge, and among the different stacking techniques, stacking based on field amplification is the most effective and simplest method of achieving high sensitivity without special complicated mechanisms or operations. This review introduces several stacking techniques based on field amplification. Field-amplified sample stacking, large-volume sample stacking, matrix field-amplified stacking injection (FASI), head-column FASI, matrix FASI combined with head-column FASI, FASI coupled with extraction and clean-up methods, electrokinetic supercharging, cation-anion selective exhaustive injection-sweeping-micellar electrokinetic chromatography, and newly developed techniques based on field amplification combined with other methods are included, and examples of straightforward methods for solving the sensitivity problem are provided. We also present a brief overview of the advantages, limitations, and future developments of these techniques.

Publication types

  • Review

MeSH terms

  • Animals
  • Electrophoresis, Capillary / instrumentation
  • Electrophoresis, Capillary / methods*
  • Electrophoresis, Capillary / trends
  • Humans
  • Proteins / chemistry*
  • Proteins / isolation & purification

Substances

  • Proteins