Investigation of bacterial and fungal diversity in tarag using high-throughput sequencing

J Dairy Sci. 2014 Oct;97(10):6085-96. doi: 10.3168/jds.2014-8360. Epub 2014 Aug 14.

Abstract

This is the first study on the bacterial and fungal community diversity in 17 tarag samples (naturally fermented dairy products) through a metagenomic approach involving high-throughput pyrosequencing. Our results revealed the presence of a total of 47 bacterial and 43 fungal genera in all tarag samples, in which Lactobacillus and Galactomyces were the predominant genera of bacteria and fungi, respectively. The number of some microbial genera, such as Lactococcus, Acetobacter, Saccharomyces, Trichosporon, and Kluyveromyces, among others, was found to vary between different samples. Altogether, our results showed that the microbial flora in different samples may be stratified by geographic region.

Keywords: bacterial diversity; fungal diversity; pyrosequencing; tarag.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Cattle
  • Computational Biology
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Female
  • Fermentation
  • Fungi / classification
  • Fungi / genetics
  • Fungi / isolation & purification*
  • High-Throughput Nucleotide Sequencing / veterinary*
  • Microbiota
  • Polymerase Chain Reaction / veterinary
  • Sequence Analysis, DNA / veterinary
  • Yogurt / microbiology*

Substances

  • DNA, Bacterial
  • DNA, Fungal