Abstract
The aberrant expression of PSMD10 has important functions in various malignancies. This study showed that PSMD10 was highly expressed and inversely correlated with the expression of miR-605 in intrahepatic cholangiocarcinoma (ICC) specimens. MiR-605 directly targeted and repressed PSMD10 expression. In addition, over-expression of miR-605 inhibited ICC cell progression both in vitro and in vivo. This effect of miR-605 on ICC cells was similar to that of PSMD10 knock-down by RNAi. Moreover, restoration of PSMD10 could reverse the phenotypic alteration caused by miR-605 in ICC cells. These results suggest a new therapeutic strategy in ICC by restoring miR-605, which is regulated by p53.
Keywords:
Apoptosis; Intrahepatic cholangiocarcinoma; Invasion; Proteasome 26S subunit non-ATPase 10; miR-605.
Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Animals
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Apoptosis
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Bile Duct Neoplasms / genetics
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Bile Duct Neoplasms / metabolism*
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Bile Duct Neoplasms / pathology
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Bile Ducts, Intrahepatic / metabolism*
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Bile Ducts, Intrahepatic / pathology
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Binding Sites
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Cell Line, Tumor
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Cell Proliferation
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Cholangiocarcinoma / genetics
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Cholangiocarcinoma / metabolism*
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Cholangiocarcinoma / pathology
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Gene Expression
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Gene Expression Regulation, Neoplastic
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Humans
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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MicroRNAs / physiology*
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Neoplasm Invasiveness
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Neoplasm Transplantation
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Promoter Regions, Genetic
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Proteasome Endopeptidase Complex / genetics*
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Proteasome Endopeptidase Complex / metabolism
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / metabolism
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RNA Interference
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Tumor Burden
Substances
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3' Untranslated Regions
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MIRN605 microRNA, human
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MicroRNAs
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PSMD10 protein, human
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Proto-Oncogene Proteins
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Proteasome Endopeptidase Complex