Abstract
We examined the expression of the mannose receptor CD206 by perivascular macrophages (PVM) in normal human and monkey brains and in brains of HIV-infected humans and of monkeys infected with simian immunodeficiency virus (SIV). Depletion of brain PVM in SIV-infected monkeys by intrathecal injection of liposome-encapsulated bisphosphonates eliminated CD206-expressing cells in the brain, confirming their perivascular location and phagocytic capacity. In vivo labeling with bromodeoxyuridine in normal uninfected and SIV-infected macaques in combination with CD206 immunostaining revealed a CD206+-to-CD206- shift within pre-existing PVM during SIV brain infection and neuroinflammation. These findings identify CD206 as a unique marker of human and macaque PVM, and underscore the utility of this marker in studying the origin, turnover and functions of these cells in AIDS.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Encephalitis / genetics
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Encephalitis / metabolism*
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Encephalitis / pathology
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Gene Expression Regulation, Viral
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HIV Infections / genetics
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HIV Infections / metabolism*
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HIV Infections / pathology
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HIV-1* / genetics
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Humans
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Lectins, C-Type / biosynthesis*
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Lectins, C-Type / genetics
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Macaca mulatta
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Macrophages / metabolism
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Macrophages / virology
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Male
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Mannose Receptor
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Mannose-Binding Lectins / biosynthesis*
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Mannose-Binding Lectins / genetics
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Phenotype
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Receptors, Cell Surface / biosynthesis*
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Receptors, Cell Surface / genetics
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Simian Acquired Immunodeficiency Syndrome / genetics
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Simian Acquired Immunodeficiency Syndrome / metabolism*
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Simian Acquired Immunodeficiency Syndrome / pathology
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Simian Immunodeficiency Virus* / genetics
Substances
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Lectins, C-Type
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Mannose Receptor
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Mannose-Binding Lectins
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Receptors, Cell Surface