A novel deletion mutation in RS1 gene caused X-linked juvenile retinoschisis in a Chinese family

Eye (Lond). 2014 Nov;28(11):1364-9. doi: 10.1038/eye.2014.196. Epub 2014 Aug 29.

Abstract

Purpose: X-linked juvenile retinoschisis (XLRS), a leading cause of juvenile macular degeneration, is characterized by a spoke-wheel pattern in the macular region of the retina and splitting of the neurosensory retina. This study aimed to identify the underlying genetic defect in a Chinese family with XLRS.

Methods: The proband underwent complete ophthalmic examinations, including fundus examination, fundus autofluorescence, and optical coherence tomography. DNA extracted from proband and his younger brother was screened for mutations in RS1 gene. The detected RS1 mutation was tested in all available family members and 200 healthy controls.

Results: Reduced visual acuity, spoke-wheel pattern at the fovea, and split retina were observed in the proband. A novel frameshift mutation c.206-207delTG in the RS1 gene, leading to a truncated protein (p.L69fs16X), was identified in the proband and his younger brother. This mutation was not found in any unaffected member or in the healthy controls. The mother of the proband was hemizygous for this mutant allele.

Conclusions: We identified a novel causative mutation of RS1 in a Chinese family with XLRS. This finding expands the mutation spectrum of RS1 and provides evidence for a phenotype-genotype study in XLRS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asian People / genetics*
  • Base Sequence*
  • Child
  • China
  • DNA Mutational Analysis
  • Eye Proteins / genetics*
  • Fluorescein Angiography
  • Frameshift Mutation*
  • Humans
  • Infant
  • Male
  • Pedigree
  • Polymerase Chain Reaction
  • Retinoschisis / genetics*
  • Sequence Deletion*
  • Tomography, Optical Coherence
  • Visual Acuity

Substances

  • Eye Proteins
  • RS1 protein, human