Influence of static magnetic fields combined with human insulin-like growth factor 1 on human satellite cell cultures

Richard Birk; J Ulrich Sommer; Dominik Haas; Anne Faber; Christoph Aderhold; Johannes D Schultz; Karl Hoermann; Jens Stern-Straeter

Influence of static magnetic fields combined with human insulin-like growth factor 1 on human satellite cell cultures

In Vivo. 2014 Sep-Oct;28(5):795-802.

Authors

Richard Birk¹, J Ulrich Sommer², Dominik Haas², Anne Faber², Christoph Aderhold², Johannes D Schultz², Karl Hoermann², Jens Stern-Straeter²

Affiliations

¹ Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany richard.birk@medma.uni-heidelberg.de.
² Department of Otolaryngology, Head and Neck Surgery, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

PMID: 25189891

Abstract

Tissue engineering represents a promising research field, targeting the creation of new functional muscle tissue in vitro. The aim of the present study was to show the influence of static magnetic fields (SMF) and insulin-like growth factor-1 (IGF1), as enhancing stimuli on human satellite cell cultures, which are preferred sources of stem cells in engineering skeletal muscle tissue. To detect effects on myogenic maturation and proliferation, AlamarBlue® proliferation, assay and semi-quantitative reverse transcription-polymerase chain reaction of following markers was performed: desmin (DES), myogenic factor-5 (MYF5), myogenic differentiation antigen-1 (MYOD1), myogenin (MYOG), myosin heavy chain (MYH) and α1 actin (ACTA1). As a distinct marker of differentiation, immunohistochemical staining and fusion index determination was performed on satellite cell cultures stimulated with IGF1 and IGF1-plus-SMF with an intensity of 80 mT. Proliferation was increased by additional SMF application to IGF1-stimulated cell cultures on the first day of myogenesis. Relative gene expression of measured markers was increased by IGF1 application in the first days of myogenesis except for ACTA1. Additional SMF application enhanced this effect. Nevertheless we were unable to demonstrate the formation of contractile muscle tissue. Immunhistochemical staining verified muscle origin and all markers were displayed.

Keywords: Skeletal muscle; differentiation static magnetic fields; insulin-like-1 growth factor; satellite cells; tissue engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / drug effects
Cell Differentiation / radiation effects
Cell Proliferation / drug effects
Cell Proliferation / radiation effects
Cells, Cultured
Gene Expression Profiling
Gene Expression Regulation / drug effects
Gene Expression Regulation / radiation effects
Humans
Insulin-Like Growth Factor I / pharmacology*
Magnetic Fields*
Satellite Cells, Skeletal Muscle / cytology
Satellite Cells, Skeletal Muscle / drug effects*
Satellite Cells, Skeletal Muscle / metabolism
Satellite Cells, Skeletal Muscle / radiation effects*

Substances

Insulin-Like Growth Factor I