Ten-eleven-translocation 2 (TET2) is downregulated in myelodysplastic syndromes

Eur J Haematol. 2015 May;94(5):413-8. doi: 10.1111/ejh.12445. Epub 2014 Oct 1.

Abstract

TET2, a member of the ten-eleven-translocation (TET) family genes that modify DNA by converting 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), is located in chromosome 4q24 and is frequently mutated in myeloid malignancies. The impact of TET2 mutation on survival outcomes is still controversial; however, functional studies have proved that it is a loss-of-function mutation that impairs myeloid cell differentiation and contributes to the phenotype of myeloid neoplasia. We, herein, aimed to investigate TET2 expression in patients with myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). A significantly decreased TET2 expression was observed in bone marrow cells from AML (n = 53) and patients with MDS (n = 64), compared to normal donors (n = 22). In MDS, TET2 expression was significantly reduced in RAEB-1/RAEB-2 compared to other WHO 2008 classifications, and a lower TET2 expression was observed at the time of MDS disease progression in four of five patients. In multivariate analysis, low TET2 expression (P = 0.03), male gender (P = 0.02), and WHO 2008 classification (P < 0.0001) were independent predictors of poorer overall survival. These results suggest that defective TET2 expression plays a role in the MDS pathophysiology and predicts survival outcomes in this disease.

Keywords: acute myeloid leukemia; myelodysplastic syndromes; ten-eleven-translocation 2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anemia, Refractory, with Excess of Blasts / diagnosis
  • Anemia, Refractory, with Excess of Blasts / genetics*
  • Anemia, Refractory, with Excess of Blasts / mortality
  • Anemia, Refractory, with Excess of Blasts / pathology
  • Anemia, Sideroblastic / diagnosis
  • Anemia, Sideroblastic / genetics*
  • Anemia, Sideroblastic / mortality
  • Anemia, Sideroblastic / pathology
  • Bone Marrow Cells / metabolism
  • Bone Marrow Cells / pathology
  • Case-Control Studies
  • Chromosomes, Human, Pair 4
  • DNA-Binding Proteins / genetics*
  • Dioxygenases
  • Down-Regulation
  • Female
  • Gene Expression
  • Humans
  • Leukemia, Myeloid, Acute / diagnosis
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / mortality
  • Leukemia, Myeloid, Acute / pathology
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Mutation
  • Prognosis
  • Proto-Oncogene Proteins / genetics*
  • Survival Analysis

Substances

  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Dioxygenases
  • TET2 protein, human