Co-expression of MET and CD47 is a novel prognosticator for survival of luminal breast cancer patients

Oncotarget. 2014 Sep 30;5(18):8147-60. doi: 10.18632/oncotarget.2385.

Abstract

Although luminal-type primary breast cancer can be efficiently treated, development of metastatic disease remains a significant clinical problem. We have previously shown that luminal-type circulating tumor cells (CTCs) co-expressing the tyrosine-kinase MET and CD47, a ligand involved in cancer cell evasion from macrophage scavenging, are able to initiate metastasis in xenografts. Here, we investigated the clinical relevance of MET-CD47 co-expression in 255 hormone receptor positive breast tumors by immunohistochemistry and found a 10.3- year mean overall-survival difference between MET-CD47 double-positive and double-negative patients (p<0.001) MET-CD47 co-expression defined a novel independent prognosticator for overall-survival by multivariate analysis (Cox proportional hazards model: HR: 4.1, p<0.002) and CD47 expression alone or in combination with MET was strongly associated with lymph node metastasis. Furthermore, flow cytometric analysis of metastatic patient blood revealed consistent presence of MET+CD47+ CTCs (range 0.8 - 33.3% of CTCs) and their frequency was associated with increased metastatic spread. Finally, primary uncultured CTCs with high MET+CD47+ content showed an enhanced capacity to initiate metastasis in mice. Detection and targeting of MET and CD47 may thus provide a rational basis for risk stratification and treatment of patients with luminal-type breast cancer.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Biomarkers, Tumor / analysis*
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / mortality
  • Breast Neoplasms / pathology
  • Breast Neoplasms / therapy
  • CD47 Antigen / analysis*
  • Carcinoma / metabolism*
  • Carcinoma / mortality
  • Carcinoma / secondary
  • Carcinoma / therapy
  • Female
  • Humans
  • Immunohistochemistry
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Middle Aged
  • Multivariate Analysis
  • Neoplastic Cells, Circulating / metabolism*
  • Neoplastic Cells, Circulating / pathology
  • Predictive Value of Tests
  • Prognosis
  • Proportional Hazards Models
  • Proto-Oncogene Proteins c-met / analysis*
  • Retrospective Studies
  • Risk Factors
  • Xenograft Model Antitumor Assays

Substances

  • Biomarkers, Tumor
  • CD47 Antigen
  • CD47 protein, human
  • MET protein, human
  • Proto-Oncogene Proteins c-met