T lymphocytes maintain structure and function of fibroblastic reticular cells via lymphotoxin (LT)-B

BMC Immunol. 2014 Sep 30:15:33. doi: 10.1186/s12865-014-0033-4.

Abstract

Background: Although a lot is known about how Fibroblastic Reticular Cells (FRCs) can regulate T lymphocytes (T cells), little is understood about whether or how T cells can regulate FRCs.

Results: This study shows that the absence of T cells inhibited the secretion of ER-TR7 by splenic FRCs, induced the structural disorder of FRCs, down-regulated the expression of the chemokine ligands CCL21 and CCL19, and weakened the homing ability of T cells to the spleen of nude mice. Transfusion of T cells from BALB/c mice restored the structure and functions of FRCs and recovered them. The expression of lymphotoxin (LT)-B was significantly downregulated in the absence of T cells from nude mice and was recovered after the transfusion of T cells. After the occlusion of the LT-B receptor, the FRCs' structure and functions were not restored by transfusion of T cells.

Conclusions: These data reveal that the absence of T cells will subject spleen FRCs to structural and functional abnormality, and weaken the homing ability of T cells to the spleen. These changes are attributed to the T-cell- derived LT-B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Blocking / pharmacology
  • Chemokines / metabolism
  • Down-Regulation
  • Female
  • Fibroblasts / cytology*
  • Lymphoid Tissue / cytology*
  • Lymphotoxin-beta / metabolism*
  • Mice, Inbred BALB C
  • Mice, Nude
  • Spleen / metabolism
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / immunology*

Substances

  • Antibodies, Blocking
  • Chemokines
  • Ltb protein, mouse
  • Lymphotoxin-beta