A preclinical murine model of hepatic metastases

J Vis Exp. 2014 Sep 27:(91):51677. doi: 10.3791/51677.

Abstract

Numerous murine models have been developed to study human cancers and advance the understanding of cancer treatment and development. Here, a preclinical, murine pancreatic tumor model of hepatic metastases via a hemispleen injection of syngeneic murine pancreatic tumor cells is described. This model mimics many of the clinical conditions in patients with metastatic disease to the liver. Mice consistently develop metastases in the liver allowing for investigation of the metastatic process, experimental therapy testing, and tumor immunology research.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Disease Models, Animal
  • Female
  • Liver Neoplasms, Experimental / secondary*
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Transplantation
  • Pancreatic Neoplasms / pathology*