Step by step manipulation of the CryoCapsule with HPM high pressure freezers

Methods Cell Biol. 2014:124:259-74. doi: 10.1016/B978-0-12-801075-4.00012-4.

Abstract

The CryoCapsule is a tool dedicated to correlative light to electron microscopy experiments. Focused on simplifying the specimen manipulation throughout the entire workflow from live-cell imaging to freeze substitution following cryofixation by high pressure freezing, we introduce here a step by step procedure to use the CryoCapsule either with the high pressure freezing machines: HPM010 or the HPM100.

Keywords: Correlative light and electron microscopy; CryoCapsule; HPM010; HPM100; High pressure freezing; Live-cell imaging.

MeSH terms

  • Antigens, CD / biosynthesis
  • Bacterial Proteins / biosynthesis
  • Cell Line, Tumor
  • Cryopreservation*
  • Humans
  • Lectins, C-Type / biosynthesis
  • Luminescent Proteins / biosynthesis
  • Mannose-Binding Lectins / biosynthesis
  • Microscopy, Electron, Transmission / instrumentation
  • Microscopy, Electron, Transmission / methods
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods
  • Pressure
  • Recombinant Fusion Proteins

Substances

  • Antigens, CD
  • Bacterial Proteins
  • CD207 protein, human
  • Lectins, C-Type
  • Luminescent Proteins
  • Mannose-Binding Lectins
  • Recombinant Fusion Proteins
  • yellow fluorescent protein, Bacteria