A search for Clostridium difficile ribotypes 027 and 078 in Brazil

Alexandre de Almeida Monteiro; Renata Neto Pires; Søren Persson; Edison Moraes Rodrigues Filho; Alessandro Comarú Pasqualotto

doi:10.1016/j.bjid.2014.08.004

A search for Clostridium difficile ribotypes 027 and 078 in Brazil

Braz J Infect Dis. 2014 Nov-Dec;18(6):672-4. doi: 10.1016/j.bjid.2014.08.004. Epub 2014 Oct 11.

Authors

Alexandre de Almeida Monteiro¹, Renata Neto Pires², Søren Persson³, Edison Moraes Rodrigues Filho⁴, Alessandro Comarú Pasqualotto⁵

Affiliations

¹ PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
² PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil.
³ Statens Serum Institut, Copenhagen, Denmark.
⁴ Grupo Hospitalar Conceição, Porto Alegre, RS, Brazil.
⁵ PPG Patologia, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil. Electronic address: acpasqualotto@hotmail.com.

Abstract

Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C. difficile polymerase chain reaction ribotypes 027/078 strains, we used high resolution melting and multiplex polymerase chain reaction. Among 116 screened patients, 11 were positive for C. difficile. The polymerase chain reaction ribotypes 027/078 strains were not identified in this study.

Keywords: Clostridium difficile; HRM; Multiplex-PCR; Toxin gene profiling.

MeSH terms

Brazil
Clostridioides difficile / genetics*
Clostridium Infections / microbiology*
Cross Infection / microbiology*
Diarrhea / microbiology*
Feces / microbiology
Humans
Polymerase Chain Reaction
Prospective Studies
Ribotyping*