Investigation of caspase-1 activity and interleukin-1β production in murine macrophage cell lines infected with Leishmania major

Mohammad Reza Mahmoudian Sani; Mehrdad Moosazadeh Moghaddam; Hossein Aghamollaei; Kazem Hassanpour; Ramezan Ali Taheri; Gholamreza Farnoosh

doi:10.1016/S1995-7645(14)60205-4

Investigation of caspase-1 activity and interleukin-1β production in murine macrophage cell lines infected with Leishmania major

Asian Pac J Trop Med. 2014 Sep:7S1:S70-3. doi: 10.1016/S1995-7645(14)60205-4.

Authors

Mohammad Reza Mahmoudian Sani¹, Mehrdad Moosazadeh Moghaddam², Hossein Aghamollaei³, Kazem Hassanpour⁴, Ramezan Ali Taheri⁵, Gholamreza Farnoosh⁶

Affiliations

¹ Department of Molecular Medicine & Genetic, Hamedan University of Medical Sciences, Hamedan, Iran.
² Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
³ Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.
⁴ Medical School, Sabzevar University of Medical Sciences, Sabzevar, Iran.
⁵ Nanobiotechnology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran.
⁶ Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: rzfarnoosh@yahoo.com.

PMID: 25312194
DOI: 10.1016/S1995-7645(14)60205-4

Abstract

Objective: To investigate the caspase-1 dependent inflammatory pathway activity and interleukin-1β (IL-1β) secretion in murine macrophage cell lines J774G8 infected with Leishmania major (L. major) using caspase-1 activity assay and ELISA.

Methods: Novy-MacNeal-Nicolle biphasic medium was applied to produce promastigote form of L. major. Metacyclic promastigotes in the stationary phase were applied to infect macrophage. Caspase-1 activity and IL-1β secretion were assessed by the CPP32/caspase-1 fluorometric protease assay and ELISA IL-1β kits, respectively, with time intervals of 6, 18 and 30 h.

Results: Our study showed an increase in caspase-1 activity and IL-1β secretion in infected samples compared to non-infected macrophages. The highest increase in IL-1β production was observed after 6 h of infection.

Conclusions: These results arise that the activation of inflammasome pathway could be one of the innate immunity pathways against L. major.

Keywords: Caspase-1 activity; Interleukin-1β; Leishmania major; Macrophage.