Host response biomarker in sepsis: suPAR detection

Methods Mol Biol. 2015:1237:241-6. doi: 10.1007/978-1-4939-1776-1_18.

Abstract

Recent studies of our group have shown that suPAR may complement APACHE II score for risk assessment in sepsis. suPAR may be measured in serum of patients by an enzyme immunosorbent assay developed by Virogates (suPARnostic™). Production of suPAR from circulating neutrophils and monocytes may be assessed after isolation of neutrophils and monocytes and ex vivo culture. This is followed by measurement of suPAR in culture supernatants.

MeSH terms

  • Biomarkers / blood
  • Gene Expression
  • Humans
  • Immunoenzyme Techniques
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Neutrophils / immunology
  • Neutrophils / metabolism*
  • Prognosis
  • Receptors, Urokinase Plasminogen Activator / blood*
  • Receptors, Urokinase Plasminogen Activator / genetics
  • Receptors, Urokinase Plasminogen Activator / immunology
  • Sepsis / diagnosis*
  • Sepsis / immunology
  • Sepsis / metabolism
  • Severity of Illness Index

Substances

  • Biomarkers
  • Receptors, Urokinase Plasminogen Activator