Target antigens for perinuclear antineutrophil cytoplasmic antibodies in Iranian patients with ulcerative colitis

Ali Bahari; Mahmoud Aarabi; Mehdi Hedayati; Ali Jarollahi; Farzad Firouzi; Rahim Aghazadeh; Mohammad Reza Zali; Zohreh Bari; Mohammad Hashemi; Abbas Esmaeilzadeh; Ali Mokhtarifar; Azita Ganji

Target antigens for perinuclear antineutrophil cytoplasmic antibodies in Iranian patients with ulcerative colitis

Middle East J Dig Dis. 2014 Oct;6(4):203-7.

Authors

Affiliations

¹ Endoscopic & Minimally Invasive Surgery Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
² Department of Inflammatory Bowel Disease, Research Center of Gastroenterology and Liver Diseases, Tehran, Iran ; Department of Biomedical & Molecular Sciences, Queen's University, Canada.
³ Clinical Laboratory, Research Center for Endocrinology and Metabolic Diseases, Tehran, Iran.
⁴ Clinical Pathology Laboratory, Mofid Children Hospital, Tehran, Iran.
⁵ Research Institute of Gastroenterology and Liver Diseases (RIGLD), Shahid Beheshti University, Tehran, Iran.
⁶ Department of Inflammatory Bowel Disease, Research Center of Gastroenterology and Liver Diseases, Tehran, Iran.
⁷ Department of Gastroenterology, Mazandaran University of Medical Sciences, Sari, Iran.
⁸ Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran ; Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
⁹ Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

PMID: 25349683
PMCID: PMC4208928

Abstract

BACKGROUND Patients with ulcerative colitis (UC) carry autoantibodies such as perinuclear antineutrophil cytoplasmic antibodies (p-ANCA).

Objective: The aim of the present study was to evaluate the target antigens for p-ANCA in Iranian patients with UC. METHODS p-ANCA target antigens including elastase, lactoferrin, cathepsin G, myeloproxidase, lysozyme, and bactericidal permeability increasing protein (BPI) were determined in 113 patients with UC using enzyme-linked immunosorbent assay (ELISA). RESULTS 59.2% of the patients were positive for at least one antigen and p-ANCA directed against lactoferrin, elastase, lysozyme, cathepsin G, Bactericidal permeability increasing protein, and myeloproxidase in 31.5%, 25.9%, 8.3%, 7.4%, 5.6%, and 0% of the patients, respectively. CONCLUSION The highest prevalence of p-ANCA was observed against lactoferrin and elastase. Also, myeloproxidase was not an antigen for p-ANCA among our patients.

Keywords: Anti-neutrophil cytoplasmic antibody; Inflammatory bowel disease; Ttarget antigen; Ulcerative colitis.