Characterization of albendazole-randomly methylated-β-cyclodextrin inclusion complex and in vivo evaluation of its antihelmitic activity in a murine model of Trichinellosis

PLoS One. 2014 Nov 18;9(11):e113296. doi: 10.1371/journal.pone.0113296. eCollection 2014.

Abstract

Albendazole is a benzimidazole carbamate extensively used in oral chemotherapy against intestinal parasites, due to its broad spectrum activity, good tolerance and low cost. However, the drug has the disadvantage of poor bioavailability due to its very low solubility in water; as a consequence, a very active area of research focuses on the development of new pharmaceutical formulations to increase its solubility, dissolution rate, and bioavailability. The primary objective of this study was to prepare randomly methylated β-cyclodextrins inclusion complexes to increase albendazole dissolution rate, in order to enhance its antiparasitic activity. This formulation therapeutic efficacy was contrasted with that of the pure drug by treating Trichinella spiralis infected mice during the intestinal phase of the parasite cycle, on days five and six post-infection. This protocol significantly decreased muscle larval burden measured in the parenteral stage on day 30 post-infection, when compared with the untreated control. Thus, it could be demonstrated that the inclusion complexes improve the in vivo therapeutic activity of albendazole.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albendazole / chemistry*
  • Albendazole / pharmacology
  • Animals
  • Antiparasitic Agents / pharmacology*
  • Calorimetry, Differential Scanning
  • Disease Models, Animal*
  • Macromolecular Substances / chemistry
  • Macromolecular Substances / pharmacology*
  • Mass Spectrometry
  • Methylation
  • Mice
  • Muscle, Skeletal / parasitology
  • Solubility
  • Trichinellosis / drug therapy*
  • X-Ray Diffraction
  • beta-Cyclodextrins / chemistry*
  • beta-Cyclodextrins / pharmacology

Substances

  • Antiparasitic Agents
  • Macromolecular Substances
  • beta-Cyclodextrins
  • Albendazole
  • betadex

Grants and funding

The work was supported by the following: Proyectos de investigación plurianuales. Funder: Consejo Nacional de Investigaciones Científicas y Técnicas, N° 112-201001-00194, MCL and DL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.