Plasma microRNAs as potential noninvasive biomarkers for in-stent restenosis

PLoS One. 2014 Nov 26;9(11):e112043. doi: 10.1371/journal.pone.0112043. eCollection 2014.

Abstract

Objective: To investigate whether microRNAs (miRs) can serve as novel biomarkers for in-stent restenosis (ISR).

Methods: This retrospective, observational single-centre study was conducted at the cardiovascular department of a tertiary hospital centre in the north of China. Follow-up coronary angiography at 6 to 12 months was performed in 181 consecutive patients implanted with drug-eluting stents. Fifty-two healthy volunteers served as the control group. The plasma miRs levels were analyzed by quantitative real-time PCR. Receiver-operating characteristic curve (ROC) analysis was performed to investigate the characters of these miRs as potential biomarkers of ISR.

Results: MiR-21 levels in ISR patients were significantly higher than those in non-ISR patients and healthy controls (P<0.05), while miR-100 (P<0.05), miR-143 (P<0.001) and miR-145 (P<0.0001) levels were significantly decreased in ISR patients. Further analysis showed that miR-21 levels were remarkably increased (P = 0.045), while miR-100 (P = 0.041), miR-143 (P = 0.029) and miR-145 (P<0.01) levels were dramatically decreased in patients with diffuse ISR compared to those with focal ISR. ROC analysis demonstrated that the area under curve of miR-145, miR-143, miR-100 and miR-21 were 0.880 (95% confidence interval; CI = 0.791-0.987, P<0.001), 0.818 (95% confidence interval; CI = 0.755-0.963, P<0.001), 0.608 (95% confidence interval; CI = 0.372-0.757, P<0.05) and 0.568 (95% confidence interval; CI = 0.372-0.757, P<0.05), with specificity of 83.1%, 80.1%, 68.9% and 68.6%, and sensitivity of 88.7%, 82.1%, 60.2% and 50.1%, respectively.

Conclusions: Circulating miR-143 and miR-145 levels are associated with the occurrence of ISR and can serve as novel noninvasive biomarkers for ISR.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Angioplasty, Balloon, Coronary*
  • Area Under Curve
  • Biomarkers / blood
  • Case-Control Studies
  • Coronary Angiography
  • Coronary Restenosis / blood*
  • Coronary Restenosis / diagnosis
  • Coronary Restenosis / diagnostic imaging
  • Drug-Eluting Stents*
  • Female
  • Humans
  • Male
  • MicroRNAs / blood*
  • Middle Aged
  • ROC Curve
  • Retrospective Studies

Substances

  • Biomarkers
  • MIRN143 microRNA, human
  • MIRN145 microRNA, human
  • MicroRNAs

Grants and funding

This work was supported by a grant of National Basic Research Program of China (973 program, 2014CB542401 to YL), Natural Science Foundation of China (No. 30971251, 81070160, 81470462, 81100121, 81100071), the Heilongjiang Province Outstanding Youth Foundation (No. JC201208), the Collegiate Changjiang Scholar Reserve Support Plan of Heilongjiang Province (No. 2013CJHB001), the grants for study abroad from the Heilongjiang Provincial Science and Technology agency (No. LC08C36). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.