Induction of caspase cascade pathway by kaempferol-3- O-rhamnoside in LNCaP prostate cancer cell lines

Biomed Rep. 2015 Jan;3(1):115-117. doi: 10.3892/br.2014.385. Epub 2014 Nov 14.

Abstract

Prostate cancer has become a leading cause of mortality in humans. Previous studies have shown the potential anticancer properties of kaempferol-3-O-rhamnoside in breast cancer cell lines. In the present study, the anticancer potential of kaempferol-3-O-rhamnoside was investigated in LNCaP human prostate cancer cell lines. The inhibition of cell proliferation was investigated using MTT assays, whereas its ability to induce the caspase-cascade pathway was investigated by western blotting. The results showed that kaempferol-3-O-rhamnoside inhibits the proliferation of LNCaP cells in a dose-dependent manner by upregulating the expression of caspase-8, caspase-9, caspase-3 and poly (ADP-ribose) polymerase proteins. Although further studies are required, the results of the present study indicate the potential application of kaempferol-3-O-rhamnoside in cancer treatment.

Keywords: LNCaP; apoptosis; cancer; caspase; kaempferol-3-O-rhamnoside.