Parafilm-assisted microdissection: a sampling method for mass spectrometry-based identification of differentially expressed prostate cancer protein biomarkers

Chem Commun (Camb). 2015 Mar 18;51(22):4564-7. doi: 10.1039/c4cc08331h.

Abstract

Mass spectrometry-based methods for prostate cancer biomarker discovery are hampered by their low-throughput capabilities because of extensive sample preparation. We present the parafilm-assisted microdissection technique coupled with label-free quantification and bioinformatics analysis as a means to evaluate directly protein expression changes on benign and tumor regions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Biomarkers, Tumor / biosynthesis
  • Computational Biology
  • Humans
  • Male
  • Mass Spectrometry*
  • Microdissection / methods*
  • Neoplasm Proteins / analysis*
  • Neoplasm Proteins / biosynthesis
  • Paraffin*
  • Prostatic Neoplasms / diagnosis*
  • Prostatic Neoplasms / metabolism*

Substances

  • Biomarkers, Tumor
  • Neoplasm Proteins
  • Paraffin