Discovering cancer biomarkers from clinical samples by protein microarrays

Proteomics Clin Appl. 2015 Feb;9(1-2):98-110. doi: 10.1002/prca.201400094.

Abstract

Cancer biomarkers are of potential use in early cancer diagnosis, anticancer therapy development, and monitoring the responses to treatments. Protein-based cancer biomarkers are major forms in use, as they are much easier to be monitored in body fluids or tissues. For cancer biomarker discovery, high-throughput techniques such as protein microarrays hold great promises, because they are capable of global unbiased monitoring but with a miniaturized format. In doing so, novel and cancer type specific biomarkers can be systematically discovered at an affordable cost. In this review, we give a relatively complete picture on protein microarrays applied to clinical samples for cancer biomarker discovery, and conclude this review with the future perspectives.

Keywords: Cancer biomarker; Cancer metastasis; Clinical samples; Protein microarray.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biomarkers, Tumor / metabolism*
  • Body Fluids / metabolism*
  • Humans
  • Neoplasm Proteins / metabolism*
  • Neoplasms / diagnosis*
  • Neoplasms / metabolism*
  • Protein Array Analysis / methods*
  • Proteome / analysis*

Substances

  • Biomarkers, Tumor
  • Neoplasm Proteins
  • Proteome