Competitive peptide antagonists of ANF-induced cyclic guanosine monophosphate production

T J Abell; A M Richards; T G Yandle; E A Espiner; C Frampton; H Ikram

doi:10.1016/0006-291x(89)91689-6

Competitive peptide antagonists of ANF-induced cyclic guanosine monophosphate production

Biochem Biophys Res Commun. 1989 Oct 16;164(1):108-13. doi: 10.1016/0006-291x(89)91689-6.

Authors

T J Abell¹, A M Richards, T G Yandle, E A Espiner, C Frampton, H Ikram

Affiliation

¹ Department of Medicine, Princess Margaret Hospital, Christchurch, New Zealand.

PMID: 2553010
DOI: 10.1016/0006-291x(89)91689-6

Abstract

Atrial natriuretic factor (isoleucine ANF 101-126), cleaved ANF (isoleucine ANF 101-105/106-126) and des (Gln 18, Ser 19, Gly 20, Leu 21, Gly 22) ANF 4-23-NH2 (C-ANF 4-23) stimulated cyclic guanosine monophosphate production (cGMP) by rat aortic vascular smooth muscle cells (VSMC) in culture. Cleaved ANF and ANF C4-23 also antagonised or diminished the response to ANF 101-126. Agonist and antagonist actions of both peptides were dose-related. In contrast, prepro ANF (104-123), an ANF precursor fragment, exhibited no agonist or antagonist effect on cGMP production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Atrial Natriuretic Factor / metabolism
Atrial Natriuretic Factor / pharmacology*
Cells, Cultured
Cyclic GMP / antagonists & inhibitors
Cyclic GMP / biosynthesis*
Cyclic GMP / metabolism
Muscle, Smooth, Vascular / cytology
Muscle, Smooth, Vascular / metabolism
Protein Precursors / metabolism
Rats
Rats, Inbred WKY

Substances

Protein Precursors
Atrial Natriuretic Factor
Cyclic GMP