Diet-induced unresolved ER stress hinders KRAS-driven lung tumorigenesis

Cell Metab. 2015 Jan 6;21(1):117-25. doi: 10.1016/j.cmet.2014.11.020. Epub 2014 Dec 18.

Abstract

Dietary effects on tumor biology can be exploited to unravel cancer vulnerabilities. Here, we present surprising evidence for anti-proliferative action of high-calorie-diet (HCD) feeding on KRAS-driven lung tumors. Tumors of mice that commenced HCD feeding before tumor onset displayed defective unfolded protein response (UPR) and unresolved endoplasmic reticulum (ER) stress. Unresolved ER stress and reduced proliferation are reversed by chemical chaperone treatment. Whole-genome transcriptional analyses revealed FKBP10 as one of the most downregulated chaperones in tumors of the HCD-pre-tumor-onset group. FKBP10 downregulation dampens tumor growth in vitro and in vivo. Providing translational value to these results, we report that FKBP10 is expressed in human KRAS-positive and -negative lung cancers, but not in healthy parenchyma. Collectively, our data shed light on an unexpected anti-tumor action of HCD imposed before tumor onset and identify FKBP10 as a putative therapeutic target to selectively hinder lung cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Transformation, Neoplastic / drug effects
  • Diet*
  • Down-Regulation
  • Doxycycline / toxicity
  • Endoplasmic Reticulum Stress / drug effects
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology*
  • Male
  • Mice
  • Mice, SCID
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism
  • Phenylbutyrates / toxicity*
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Tacrolimus Binding Proteins / antagonists & inhibitors
  • Tacrolimus Binding Proteins / genetics
  • Tacrolimus Binding Proteins / metabolism
  • Transplantation, Heterologous
  • Unfolded Protein Response / drug effects
  • ras Proteins / genetics
  • ras Proteins / metabolism*

Substances

  • Molecular Chaperones
  • Phenylbutyrates
  • RNA, Small Interfering
  • 4-phenylbutyric acid
  • ras Proteins
  • Tacrolimus Binding Proteins
  • Fkbp10 protein, mouse
  • Doxycycline