Accuracy of different methods for susceptibility testing of gentamicin with KPC carbapenemase-producing Klebsiella pneumoniae

Diagn Microbiol Infect Dis. 2015 Feb;81(2):132-4. doi: 10.1016/j.diagmicrobio.2014.10.011. Epub 2014 Nov 4.

Abstract

Performance of Vitek2, Etest, and TREK broth microdilution (BMD) panels was evaluated versus reference CLSI BMD for gentamicin susceptibility testing with 57 bloodstream isolates of KPC-producing Klebsiella pneumoniae. Compared with reference BMD, the Essential Agreement and Categorical Agreement for TREK panels, Vitek2, and Etest were 91.2%, 31.6%, and 61.4%, respectively, and 86%, 21%, and 52.6%, respectively. Four very major discrepancies occurred with Vitek2. In these 4 strains, gentamicin resistance was associated with the presence of an armA aminoglycoside resistance determinant.

Keywords: Aminoglycosides; Automated system; Broth microdilution; Carbapenem-resistant; Etest; Klebsiella pneumoniae; Vitek2.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Automation, Laboratory / methods
  • Bacteremia / microbiology
  • Bacterial Proteins / metabolism*
  • Diagnostic Errors*
  • Gentamicins / pharmacology*
  • Humans
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / enzymology*
  • Microbial Sensitivity Tests / methods
  • beta-Lactamases / metabolism*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Gentamicins
  • beta-Lactamases
  • carbapenemase