Detection of blaCTX-M-type genes in complex class 1 integrons carried by Enterobacteriaceae isolated from retail chicken meat in Brazil

Int J Food Microbiol. 2015 Mar 16:197:88-91. doi: 10.1016/j.ijfoodmicro.2014.12.001. Epub 2014 Dec 11.

Abstract

CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have been increasingly identified in humans and animals, and their potential transmission by contaminated food has been highlighted. In this study, we report for the first time the isolation of multidrug-resistant (MDR) Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis strains harboring blaCTXM-2 or blaCTXM-8 gene variants in chicken meat sold in markets in southeast Brazil. In this regard, the genetic environment of the blaCTX-M-2 gene is composed of a complex class 1 integron and an ISCR1-associated sequence with dfr and/or aadA gene cassettes located within the variable region. In summary, chicken meat may be a reservoir of MDR Enterobacteriaceae harboring blaCTX-M-type genes, which is a public health concern.

Keywords: CTX-M; Chicken meat; ISCR1; Poultry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brazil
  • Chickens
  • Drug Resistance, Multiple / genetics
  • Enterobacteriaceae / genetics*
  • Enterobacteriaceae / isolation & purification
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Food Microbiology*
  • Integrons / genetics*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / isolation & purification
  • Meat / microbiology*
  • Proteus mirabilis / genetics
  • Proteus mirabilis / isolation & purification
  • beta-Lactamases / genetics*

Substances

  • beta-Lactamases