Sequence and regulation of the porcine FSHR gene promoter

Wangjun Wu; Jing Han; Rui Cao; Jinbi Zhang; Bojiang Li; Zequn Liu; Kaiqing Liu; Qifa Li; Zengxiang Pan; Jie Chen; Honglin Liu

doi:10.1016/j.anireprosci.2014.11.023

Sequence and regulation of the porcine FSHR gene promoter

Anim Reprod Sci. 2015 Mar:154:95-104. doi: 10.1016/j.anireprosci.2014.11.023. Epub 2015 Jan 8.

Authors

Wangjun Wu¹, Jing Han², Rui Cao³, Jinbi Zhang⁴, Bojiang Li⁵, Zequn Liu⁶, Kaiqing Liu⁷, Qifa Li⁸, Zengxiang Pan⁹, Jie Chen¹⁰, Honglin Liu¹¹

Affiliations

¹ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wuwangjun2009@hotmail.com.
² Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: linzog@yahoo.com.cn.
³ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2011105017@njau.edu.cn.
⁴ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: Jinbi.zhang@auckland.ac.nz.
⁵ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2012105021@njau.edu.cn.
⁶ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liuzequn0806@hotmail.com.
⁷ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2012105020@njau.edu.cn.
⁸ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liqifa@njau.edu.cn.
⁹ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: owwa@njau.edu.cn.
¹⁰ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: jiechen@njau.edu.cn.
¹¹ Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liuhonglin@njau.edu.cn.

PMID: 25599592
DOI: 10.1016/j.anireprosci.2014.11.023

Abstract

Follicle-stimulating hormone (FSH) plays a crucial role in animal reproduction and exerts its physiological functions by interacting with the FSH receptor (FSHR). The FSHR is exclusively expressed in granulose cells in the ovary and its expression level is closely related to granulose cell differentiation and follicle maturation. In mammal, most of the follicles undergo atresia, while follicle atresia is mainly caused by granulosa cell apoptosis. However, knowledge on the transcriptional regulatory mechanisms of the porcine FSHR gene in granulosa cell is still limited. In this study, approximately 2.1kb of the proximal promoter sequence of the porcine FSHR gene were obtained by genome walking, and the regulatory elements and transcription factors in the porcine FSHR promoter sequence were predicted. Furthermore, the core promoter region (-1195/-598) of the porcine FSHR gene was identified using a luciferase assay. Subsequently, the relationship between expression levels of the porcine FSHR gene and histone H3K9 acetylation levels around the core promoter region (-787/-572) in vivo and invitro were analyzed. Our results showed that an increased FSHR gene expression level was accompanied with an increase in histone H3K9 acetylation levels, suggesting that histone H3K9 acetylation could regulate the expression of the porcine FSHR gene.

Keywords: FSHR; Granulosa cells; Histone acetylation; Pig; Transcription.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Chromosome Walking
Cloning, Molecular
Follicle Stimulating Hormone / genetics
Follicle Stimulating Hormone / metabolism*
Gene Expression Regulation
Histones / metabolism
Molecular Sequence Data
Promoter Regions, Genetic
Receptors, FSH / genetics
Receptors, FSH / metabolism*
Swine* / genetics
Swine* / metabolism

Substances

Histones
Receptors, FSH
Follicle Stimulating Hormone