Abstract
The cis vs trans conformation, or shape, of phosphoserine-proline (pSer-Pro), a prevalent motif in cell cycle proteins, may play a significant role in regulating mitosis. We demonstrate that Cdk1-cyclin B, the central mitotic kinase, is specific for the trans conformation, not cis, of synthetic, locked Ser-Pro 11-residue peptide substrates, using LC-MSMS detection and sequencing of phosphorylated products. This substrate stereospecificity may contribute an additional level of mitotic regulation.
Publication types
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Letter
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Research Support, N.I.H., Extramural
MeSH terms
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CDC2 Protein Kinase
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Cyclin B1 / chemistry*
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Cyclin B1 / metabolism*
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Cyclin-Dependent Kinases / chemistry*
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Cyclin-Dependent Kinases / metabolism*
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Mitosis
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NIMA-Interacting Peptidylprolyl Isomerase
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Peptides / chemistry
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Peptides / metabolism
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Peptidylprolyl Isomerase / metabolism
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Phosphorylation
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Protein Conformation
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Solid-Phase Synthesis Techniques
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Stereoisomerism
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Tandem Mass Spectrometry
Substances
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CCNB1 protein, human
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Cyclin B1
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NIMA-Interacting Peptidylprolyl Isomerase
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Peptides
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CDC2 Protein Kinase
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CDK1 protein, human
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Cyclin-Dependent Kinases
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PIN1 protein, human
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Peptidylprolyl Isomerase