Methods to generate genetically engineered mouse models of soft tissue sarcoma

Methods Mol Biol. 2015:1267:283-95. doi: 10.1007/978-1-4939-2297-0_13.

Abstract

We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Cell Separation
  • Cell Transformation, Neoplastic
  • Disease Models, Animal
  • Genetic Engineering / methods*
  • Integrases / metabolism
  • Mice
  • Mice, Transgenic
  • Mutation
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Sarcoma / genetics*
  • Sarcoma / pathology
  • Tamoxifen / pharmacology
  • Tumor Suppressor Protein p53 / genetics

Substances

  • Tumor Suppressor Protein p53
  • Tamoxifen
  • Cre recombinase
  • Integrases
  • Hras protein, mouse
  • Proto-Oncogene Proteins p21(ras)