Association of miR-1266 with recurrence/metastasis potential in estrogen receptor positive breast cancer patients

Asian Pac J Cancer Prev. 2015;16(1):291-7. doi: 10.7314/apjcp.2015.16.1.291.

Abstract

The Homeobox B13 (HOXB13):Interleukin 17 Receptor B (IL17BR) index of estrogen receptor (ER)-positive breast cancer (ER (+) BC) patients may be a potential biomarker of recurrence/ metastasis. However, effects of microRNA (miRNA) binding to the 3' untranslated region (3 ?UTR) of HOXB13 and IL17BR and its function on recurrence/metastasis in ER (+) BC remains elusive. The aims of this study were to determine the expression of miRNAs that bind to 3 ?UTR of HOXB13 and IL17BR in ER (+) BC patients and asess the effects of these miRNAs on recurrence/metastasis. The expression profiles of HOXB13 and IL17BR were evaluated using RT- PCR in tumors and normal tissue samples from 40 ER (+) BC patients. The expression level of 4 miRNAs, which were predicted to bind the 3 ?UTR of HOXB13 and IL17BR using TargetScan, microRNA.org and miRDB online databases, were further evaluated with RT-PCR. Our findings demonstrated that high miR-1266 levels might be significant prognostic factor for recurrence/metastasis occurrence (3.05 fold p=0.004) and tamoxifen response (3.90 fold; p=0.2514) in ER (+) BC cases. Although we suggest that modulation of miR-1266 expression may be an important mechanism underlying the chemoresistance of ER (+) BC, advanced studies and validation are required.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Adult
  • Aged
  • Aged, 80 and over
  • Antineoplastic Agents, Hormonal / therapeutic use
  • Biomarkers, Tumor / genetics
  • Breast Neoplasms, Male / drug therapy
  • Breast Neoplasms, Male / genetics*
  • Drug Resistance, Neoplasm
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Homeodomain Proteins / genetics
  • Humans
  • Male
  • MicroRNAs / biosynthesis
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Metastasis / genetics*
  • Neoplasm Recurrence, Local / genetics*
  • RNA, Messenger / biosynthesis
  • Receptors, Estrogen / metabolism*
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin-17
  • Tamoxifen / therapeutic use

Substances

  • 3' Untranslated Regions
  • Antineoplastic Agents, Hormonal
  • Biomarkers, Tumor
  • HOXB13 protein, human
  • Homeodomain Proteins
  • IL17RB protein, human
  • MIRN1266 microRNA, human
  • MicroRNAs
  • RNA, Messenger
  • Receptors, Estrogen
  • Receptors, Interleukin
  • Receptors, Interleukin-17
  • Tamoxifen