Modulating Vibrio cholerae quorum-sensing-controlled communication using autoinducer-loaded nanoparticles

Nano Lett. 2015 Apr 8;15(4):2235-41. doi: 10.1021/acs.nanolett.5b00151. Epub 2015 Mar 3.

Abstract

The rise of bacterial antibiotic resistance has created a demand for alternatives to traditional antibiotics. Attractive possibilities include pro- and anti-quorum sensing therapies that function by modulating bacterial chemical communication circuits. We report the use of Flash NanoPrecipitation to deliver the Vibrio cholerae quorum-sensing signal CAI-1 ((S)-3-hydroxytridecan-4-one) in a water dispersible form as nanoparticles. The particles activate V. cholerae quorum-sensing responses 5 orders of magnitude higher than does the identically administered free CAI-1 and are diffusive across in vivo delivery barriers such as intestinal mucus. This work highlights the promise of combining quorum-sensing strategies with drug delivery approaches for the development of next-generation medicines.

Keywords: CAI-1; Nanoparticle; Vibrio cholerae; autoinducer; drug delivery; quorum sensing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biofilms / drug effects*
  • Biofilms / growth & development*
  • Ketones / administration & dosage*
  • Ketones / chemistry
  • Nanocapsules / chemistry*
  • Nanocapsules / ultrastructure
  • Particle Size
  • Quorum Sensing / drug effects*
  • Quorum Sensing / physiology*

Substances

  • 3-hydroxytridecan-4-one
  • Ketones
  • Nanocapsules