Enhanced bioactivity of Mg-Nd-Zn-Zr alloy achieved with nanoscale MgF2 surface for vascular stent application

ACS Appl Mater Interfaces. 2015 Mar 11;7(9):5320-30. doi: 10.1021/am5086885. Epub 2015 Feb 27.

Abstract

Magnesium (Mg) alloys have revolutionized the application of temporary load-bearing implants as they meet both engineering and medical requirements. However, rapid degradation of Mg alloys under physiological conditions remains the major obstacle hindering the wider use of Mg-based implants. Here we developed a simple method of preparing a nanoscale MgF2 film on Mg-Nd-Zn-Zr (denoted as JDBM) alloy, aiming to reduce the corrosion rate as well as improve the biological response. The corrosion rate of JDBM alloy exposed to artificial plasma is reduced by ∼20% from 0.337 ± 0.021 to 0.269 ± 0.043 mm·y(-1) due to the protective effect of the MgF2 film with a uniform and dense physical structure. The in vitro cytocompatibility test of MgF2-coated JDBM using human umbilical vein endothelial cells indicates enhanced viability, growth, and proliferation as compared to the naked substrate, and the MgF2 film with a nanoscale flakelike feature of ∼200-300 nm presents a much more favorable environment for endothelial cell adhesion, proliferation, and alignment. Furthermore, the animal experiment via implantation of MgF2-coated JDBM stent to rabbit abdominal aorta confirms excellent tissue compatibility of the well re-endothelialized stent with no sign of thrombogenesis and restenosis in the stented vessel.

Keywords: cytocompatibility; endothelialization; in vitro degradation; magnesium alloy; surface modification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys / chemistry*
  • Alloys / therapeutic use
  • Alloys / toxicity
  • Animals
  • Aorta, Abdominal / diagnostic imaging
  • Aorta, Abdominal / pathology
  • Biocompatible Materials / chemistry*
  • Biocompatible Materials / therapeutic use
  • Biocompatible Materials / toxicity
  • Cell Adhesion / drug effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Electrochemical Techniques
  • Fluorides / chemistry*
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Magnesium Compounds / chemistry*
  • Microscopy, Fluorescence
  • Prostheses and Implants
  • Rabbits
  • Stents*
  • Surface Properties
  • Ultrasonography

Substances

  • Alloys
  • Biocompatible Materials
  • Magnesium Compounds
  • Mg-Nd-Zn-Zr alloy
  • magnesium fluoride
  • Fluorides