Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells

Yue Tang; Yongchun Cui; Fuliang Luo; Xiaopeng Liu; Xiaojuan Wang; Aili Wu; Junwei Zhao; Zhong Tian; Like Wu

doi:10.3969/j.issn.1673-5374.2012.14.009

Cell viability and dopamine secretion of 6-hydroxydopamine-treated PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells

Neural Regen Res. 2012 May 15;7(14):1101-5. doi: 10.3969/j.issn.1673-5374.2012.14.009.

Authors

Yue Tang¹, Yongchun Cui¹, Fuliang Luo¹, Xiaopeng Liu¹, Xiaojuan Wang², Aili Wu¹, Junwei Zhao¹, Zhong Tian¹, Like Wu²

Affiliations

¹ State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China.
² Beijing Wu Stem Cells Medical Center, Beijing 100070, China.

PMID: 25722701
PMCID: PMC4340024
DOI: 10.3969/j.issn.1673-5374.2012.14.009

Abstract

In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson's Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify whether the mechanism of action relates to abnormal α-synuclein accumulation in cells. Results showed that co-culture with bone marrow-derived mesenchymal stem cells enhanced PC12 cell viability and dopamine secretion in a cell dose-dependent manner. MitoLight staining was used to confirm that PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells demonstrate reduced levels of cell apoptosis. Immunocytochemistry and western blot analysis found the quantity of α-synuclein accumulation was significantly reduced in PC12 cell and bone marrow-derived mesenchymal stem cell co-cultures. These results indicate that bone marrow-derived mesenchymal stem cells can attenuate 6-hydroxydopamine-induced cytotoxicity by reducing abnormal α-synuclein accumulation in PC12 cells.

Keywords: 6-hydroxydopamine; PC12 cells; bone marrow-derived mesenchymal stem cells; cell apoptosis; dopamine; neural regeneration; neurotoxicity; α-synuclein.