Immunological and molecular biological identification of a true case of T-hairy cell leukaemia

Eur J Haematol. 1989 Oct;43(4):339-45. doi: 10.1111/j.1600-0609.1989.tb00309.x.

Abstract

A hairy cell leukaemia (HCL) patient is presented in whom the peripheral blood mononuclear cells (PBMCs) carried suppressor T-cell markers (CD3+, CD2+, CD8+/CD4-, CD38+). Analysis of genomic DNA of PBMNC showed the presence of a monoclonal population of T cells, the T-cell receptor (TCR) beta-chain gene being rearranged on both alleles (DR/DR), while the immunoglobulin (Ig) heavy chain-genes were in germline configuration. The neoplastic cells were found to react with the monoclonal antibody RAB-1 - originally described as belonging to the B lineage-restricted monoclonal antibodies - and to carry RAB-1/CD-8 in a double marker assay. Natural killer activity of PBMNCs against K562 target cells was severely reduced, while the cells were found to exert strong antibody-dependent cellular cytotoxicity.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antibody-Dependent Cell Cytotoxicity
  • Antigens, CD / analysis
  • Deltaretrovirus Antibodies / analysis
  • Female
  • Gene Rearrangement, B-Lymphocyte
  • Gene Rearrangement, T-Lymphocyte
  • Humans
  • Killer Cells, Natural / immunology
  • Leukemia, Hairy Cell / immunology*
  • Leukemia, Hairy Cell / pathology
  • Middle Aged
  • T-Lymphocytes / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Deltaretrovirus Antibodies