Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty

Tomeu Viver; Ana Cifuentes; Sara Díaz; Gustavo Rodríguez-Valdecantos; Bernardo González; Josefa Antón; Ramon Rosselló-Móra

doi:10.1016/j.syapm.2015.02.002

Diversity of extremely halophilic cultivable prokaryotes in Mediterranean, Atlantic and Pacific solar salterns: Evidence that unexplored sites constitute sources of cultivable novelty

Syst Appl Microbiol. 2015 Jun;38(4):266-75. doi: 10.1016/j.syapm.2015.02.002. Epub 2015 Feb 20.

Authors

Tomeu Viver¹, Ana Cifuentes², Sara Díaz², Gustavo Rodríguez-Valdecantos³, Bernardo González³, Josefa Antón⁴, Ramon Rosselló-Móra²

Affiliations

¹ Marine Microbiology Group, Department of Ecology and Marine Resources, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), Esporles, Spain. Electronic address: tviver@imedea.uib-csic.es.
² Marine Microbiology Group, Department of Ecology and Marine Resources, Mediterranean Institute for Advanced Studies (IMEDEA, CSIC-UIB), Esporles, Spain.
³ Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez-Center for Applied Ecology and Sustainability, Santiago de Chile, Chile.
⁴ Department of Physiology, Genetics and Microbiology, and Multidisciplinary Institute for Environmental Studies Ramon Margalef, University of Alicante, Alicante, Spain.

PMID: 25752803
DOI: 10.1016/j.syapm.2015.02.002

Abstract

The culturable fraction of aerobic, heterotrophic and extremely halophilic microbiota retrieved from sediment and brine samples of eight sampling sites in the Mediterranean, Canary Islands and Chile was studied by means of a tandem approach combining large-scale cultivation, MALDI-TOF MS targeting whole cell biomass, and phylogenetic reconstruction based on 16S rRNA gene analysis. The approach allowed the identification of more than 4200 strains and a comparison between different sampling sites. The results indicated that the method constituted an excellent tool for the discovery of taxonomic novelty. Four new genera and nine new species could be identified within the archaeal family Halobacteriaceae, as well as one new bacterial species, and a representative of Salinibacter ruber phylotype II, a group that had been refractory to isolation for the last fifteen years. Altogether, the results indicated that in order to provide better yields for the retrieval of novel taxa from the environment, performance of non-redundant environment sampling is recommended together with the screening of large sets of strains.

Keywords: Halophilic; Large-scale cultivation; MALDI-TOF MS; OPUs; OTUs; Salterns.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Halobacteriales* / classification
Halobacteriales* / genetics
Oceans and Seas*
Phylogeny
Seawater / microbiology*