Organization of TNIK in dendritic spines

J Comp Neurol. 2015 Sep 1;523(13):1913-24. doi: 10.1002/cne.23770. Epub 2015 Jul 1.

Abstract

Tumor necrosis factor receptor-associated factor 2 (TRAF2)- and noncatalytic region of tyrosine kinase (NCK)-interacting kinase (TNIK) has been identified as an interactor in the psychiatric risk factor, Disrupted in Schizophrenia 1 (DISC1). As a step toward deciphering its function in the brain, we performed high-resolution light and electron microscopic immunocytochemistry. We demonstrate here that TNIK is expressed in neurons throughout the adult mouse brain. In striatum and cerebral cortex, TNIK concentrates in dendritic spines, especially in the vicinity of the lateral edge of the synapse. Thus, TNIK is highly enriched at a microdomain critical for glutamatergic signaling.

Keywords: PSD; RRID:AB_11213019; RRID:AB_1858225; RRID:AB_94396; RRID:nif-0000-30467; TNIK; cerebral cortex; dendritic spine; striatum RRID:AB_11212843.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology*
  • Brain / metabolism
  • Choline O-Acetyltransferase / metabolism
  • Dendritic Spines / genetics
  • Dendritic Spines / metabolism*
  • Dendritic Spines / ultrastructure
  • Gene Expression Regulation / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Immunoelectron
  • Neurons / cytology*
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism*
  • Protein Serine-Threonine Kinases / ultrastructure
  • Vesicular Glutamate Transport Protein 1 / metabolism
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Slc17a7 protein, mouse
  • Vesicular Glutamate Transport Protein 1
  • gamma-Aminobutyric Acid
  • Choline O-Acetyltransferase
  • Protein Serine-Threonine Kinases
  • TNIK protein, mouse