Abstract
The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldehydes / analysis*
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Aldehydes / chemistry
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Animals
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Biosensing Techniques / methods*
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Optical Fibers*
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Receptors, Odorant / chemistry*
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Surface Plasmon Resonance / methods*
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Sus scrofa
Substances
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Aldehydes
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Receptors, Odorant
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odorant-binding protein
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butyraldehyde
Grants and funding
This project was realized within the CNR Commessa “Progettazione e Sviluppo di Biochip per la Sicurezza Alimentare e Salute Umana”, the CISIA project and the Cluster CLAN “Safe&Smart”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.